Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Initial rates of cadmium uptake by E. coli and L. plantarum in APT medium a

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques:

Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Induction of the synthesis of mntA mRNA by Mn2+ starvation in L. plantarum ATCC 14917. Total RNA was isolated from L. plantarum ATCC 14917 Mn2+-starved (−) or Mn2+-sufficient (+) cells, treated with DNase I, and reverse transcribed with a specific antisense primer. The RT reaction mixture was used as a template to perform PCR to amplify a 1.1-kb fragment of mntA as described in the text. Each lane represents a separate experiment with four independent RNA preparations. MW, molecular weight.

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques: Isolation, Reverse Transcription, Molecular Weight

Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Identification of MntA in wild-type L. plantarum strains (A) and Mn2+-dependent mutants (B). Western blotting was done with an MntA antibody and protein extracts from L. plantarum strains and Cd2+ uptake mutants. (A) Lane 1, molecular mass standards (in kilodaltons); lanes 2 and 5, membrane protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 3 and 6, total protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 4 and 7, total protein from Mn2+-sufficient cells of ATCC 8014 and ATCC 14917, respectively. (B) Lanes 1 and 6, total protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 2 and 4, total protein from Mn2+-starved cells of mnd15-26 and mnd11-06, respectively; lanes 3 and 7, molecular weight standards; lane 5, membrane protein from Mn2+-starved cells of ATCC 8014.

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques: Western Blot, Membrane, Molecular Weight

Journal: Pharmaceuticals (Basel, Switzerland)

Article Title: The Potential of Lactiplantibacillus plantarum ATCC 14917 in the Development of Alginate-Based Gel Formulations with Anti- Staphylococcus aureus Properties.

doi: 10.3390/ph16081112

Figure Lengend Snippet: Figure 1. Antagonistic activity of selected LAB against Staphylococcus aureus 25923. **** p < 0.0001.

Article Snippet: Initially, the antagonistic actions of three lactic acid bacteria (LAB) (Lacticaseibacillus rhamnosus ATCC 10863, Lactiplantibacillus plantarum ATCC 14917, Limosilactobacillus fermentum ATCC 23271) were evaluated against S. aureus ATCC 25923.

Techniques: Activity Assay

Journal: bioRxiv

Article Title: Microbiota-Based Interventions Differentially Rescue Gut and Social Behavior Phenotypes in a Drosophila Autism-like Model

doi: 10.64898/2026.01.09.698713

Figure Lengend Snippet: Young adult control and Kdm5 LOF flies (5–7 days old) were treated with 5% sucrose (mock control), Lactiplantibacillus plantarum (Lp), Lactobacillus helveticus (Lh), or a 1:1 combination of Lp and Lh in 5% sucrose for 2–3 days, supplemented on standard fly food. Guts were dissected, homogenized and plated on selective agar media to quantify CFUs per gut of Acetobacter (A) , Enterobacter (B) and Lactobacilli (C) genera. Bars represent mean ± SEM from three independent biological replicates, with duplicate technical platings per sample. Statistical significance was determined by one-way ANOVA followed by Dunnetts multiple-comparisons test. **p < 0.01; ****p < 0.0001; ns, not significant.

Article Snippet: The probiotics used were Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum ) (ATCC 14917) and Lactobacillus helveticus (ATCC 15009), both obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Journal: bioRxiv

Article Title: Microbiota-Based Interventions Differentially Rescue Gut and Social Behavior Phenotypes in a Drosophila Autism-like Model

doi: 10.64898/2026.01.09.698713

Figure Lengend Snippet: Young adult control and Kdm5 LOF flies (5–7 days old) were treated with 5% sucrose (mock control), Lactiplantibacillus plantarum (Lp), Lactobacillus helveticus (Lh), or a 1:1 combination of Lp and Lh in 5% sucrose for 2–3 days, prior to behavioral testing. Social spacing behavior was assessed using the Social Distance assay. Histograms (A–H) show the percentage of flies occupying 0.5-cm distance bins, calculated based on the distance from the nearest to the farthest neighbor. Panels A–D correspond to control flies, and panels E–H correspond to Kdm5 LOF flies under the indicated treatment conditions. (I) The Social Space Index (SSI) was calculated as the percentage of flies in the first distance bin minus the percentage in the second bin for each condition. Positive SSI values indicate increased social proximity, whereas values at or below zero indicate reduced social interaction. Bars represent mean ± SEM from three independent biological replicates. Statistical significance was determined using a two-way ANOVA followed by Šídák’s multiple-comparisons test. All post hoc comparisons were performed within genotypes; only the two statistically significant comparisons are indicated (**p < 0.01; ***p < 0.001).

Article Snippet: The probiotics used were Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum ) (ATCC 14917) and Lactobacillus helveticus (ATCC 15009), both obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Journal: bioRxiv

Article Title: Microbiota-Based Interventions Differentially Rescue Gut and Social Behavior Phenotypes in a Drosophila Autism-like Model

doi: 10.64898/2026.01.09.698713

Figure Lengend Snippet: Young adult control (gut-specific Lucierase RNAi ) and gut-specific Kdm5 RNAi flies (5-7 days old; RNAi induced at 29°C for 3 days) were treated with 5% sucrose (mock control), L. plantarum ( Lp ), L. helveticus ( Lh ) or a 1:1 combination of Lp and Lh in 5% sucrose for 2-3 days, supplemented on standard fly food. Guts were dissected, homogenized and plated on selective agar media to quantify CFUs per gut of Acetobacter (A) , Enterobacter (B) and Lactobacilli (C) genera. Bars represent mean ± SEM from three independent biological replicates, with duplicate technical platings per sample. Statistical significance was determined by one-way ANOVA followed by Dunnett’s or Šídák’s multiple-comparisons test, as indicated. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant.

Article Snippet: The probiotics used were Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum ) (ATCC 14917) and Lactobacillus helveticus (ATCC 15009), both obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Journal: bioRxiv

Article Title: Microbiota-Based Interventions Differentially Rescue Gut and Social Behavior Phenotypes in a Drosophila Autism-like Model

doi: 10.64898/2026.01.09.698713

Figure Lengend Snippet: Young adult control (gut-specific Lucierase RNAi ) and gut-specific Kdm5 RNAi flies (5-7 days old; (5-7 days old; RNAi induced at 29°C for 3 days) were treated with 5% sucrose (mock control), L. plantarum ( Lp ), L. helveticus ( Lh ) or a 1:1 combination of Lp and Lh in 5% sucrose for 2-3 days prior to behavioral testing. Social spacing behavior was assessed using the social distance assay. Histograms (A–H) show the percentage of flies occupying 0.5-cm distance bins, calculated based on the distance from each fly to its nearest neighbor. Panels A–D correspond to control flies, and panels E–H correspond to gut-specific Kdm5 RNAi flies under the indicated treatment conditions. (I) The Social Space Index (SSI) was calculated as the percentage of flies in the first distance bin minus the percentage in the second bin for each condition. Positive SSI values indicate increased social proximity, whereas SSI values ≤ 0 indicate reduced social interaction. Bars represent mean ± SEM from three independent biological replicates. Statistical significance was determined using a two-way ANOVA followed by Šídák’s multiple-comparisons test; no post hoc SSI comparisons reached statistical significance.

Article Snippet: The probiotics used were Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum ) (ATCC 14917) and Lactobacillus helveticus (ATCC 15009), both obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Journal: bioRxiv

Article Title: Microbiota-Based Interventions Differentially Rescue Gut and Social Behavior Phenotypes in a Drosophila Autism-like Model

doi: 10.64898/2026.01.09.698713

Figure Lengend Snippet: Differential abundance analysis was performed on 16S rRNA gene sequencing data from recipient flies following FMT. (A) Coefficient plot showing differential abundance of selected bacterial taxa across donor–recipient combinations following FMT. Positive coefficient values indicate relative enrichment, whereas negative values indicate relative depletion. (B–C) Relative abundance of Lactobacillus plantarum (B) and Lactobacillus pentosus–plantarum (C) in control and Kdm5 LOF recipient flies following FMT. Individual points represent biological replicates. Each condition represents three independent biological replicates. Statistical significance was determined using differential abundance analysis with false discovery rate (FDR) correction. Adjusted p-values and coefficients are indicated where significant.

Article Snippet: The probiotics used were Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum ) (ATCC 14917) and Lactobacillus helveticus (ATCC 15009), both obtained from the American Type Culture Collection (ATCC).

Techniques: Sequencing, Control